Disease:

Colorectal adenocarcinoma, Dukes' type C 

Origin:

 Derived from metastatic lymph node 

Species:

Homo sapiens 

Tissue:

Colon 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

Tumorigenic; The stemline chromosome number is hyperdiploid 

Patient:

Male, Caucasian, 51 yrs of age 

Medium:

L-15 + 10% FBS 

Subculture:

1:2 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:
 I 
Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:

Isoenzymes:
 ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 2 PGM3, 1
DNA Profile (STR):
 Amelogenin: X CSF1PO: 13,14 D13S317: 12 D16S539: 9,13 D5S818: 13 D7S820: 8,9 THO1: 8 TPOX: 11 vWA: 16 
Mutations in SW620 Cells

Related cells by Organ:

Caco-2 cells 

DLD-1 cells 

HCA-7 cells 

HCT-116 cells 

SW480 cells 

HT-29 cells 

LS-180 cells 

SW620 cells 

T84 cells 

SW48 cells 

RKO cells 

SNU-407 cells 

SNU-503 cells 

LS-174T cells 

LoVo cells 

HCT15 cells 


References for SW620 cells:

1. Chen, X. Q., Wu, J. B., Yang, X. Q., Song, Y. G., and Li, M. S. [Effects of epigallocatechin-3-gallate on the proliferation of colonic cancer cell line SW620 and PAK1 gene expression]. Nan Fang Yi Ke Da Xue Xue Bao, 29: 1568-1570, 2009. 

2. Kong, C. K., Lam, W. S., Chiu, L. C., Ooi, V. E., Sun, S. S., and Wong, Y. S. A rice bran polyphenol, cycloartenyl ferulate, elicits apoptosis in human colorectal adenocarcinoma SW480 and sensitizes metastatic SW620 cells to TRAIL-induced apoptosis. Biochem Pharmacol, 77: 1487-1496, 2009. 

3. Liu, Y. D., Wang, S. M., Huang, Z. H., and Li, Q. [Effect of KDR recombinant adenovirus containing double suicide gene on the proliferation of human colon adneocarcinoma SW620 cells]. Nan Fang Yi Ke Da Xue Xue Bao, 29: 887-889, 893, 2009. 

4. Matuo, R., Sousa, F. G., Escargueil, A. E., Grivicich, I., Garcia-Santos, D., Chies, J. A., Saffi, J., Larsen, A. K., and Henriques, J. A. 5-Fluorouracil and its active metabolite FdUMP cause DNA damage in human SW620 colon adenocarcinoma cell line. J Appl Toxicol, 29: 308-316, 2009. 

5. Rudolf, E., Andelova, H., and Cervinka, M. Activation of several concurrent proapoptic pathways by sulforaphane in human colon cancer cells SW620. Food Chem Toxicol, 47: 2366-2373, 2009. 

6. Storvold, G. L., Fleten, K. G., Olsen, C. G., Follestad, T., Krokan, H. E., and Schonberg, S. A. Docosahexaenoic acid activates some SREBP-2 targets independent of cholesterol and ER stress in SW620 colon cancer cells. Lipids, 44: 673-683, 2009.