糖酵解实际上在几乎所有的原核细胞和真核细胞中都有发现。这一途径在肿瘤细胞中高度上调(瓦博格效应--Warburg effect)。参与糖酵解的酶是癌症治疗的潜在目标。BioVision提供实验测定糖酵解途径的各种代谢物,辅助因子和酶的一系列测定产品。
试剂盒特点:
非放射性,均质检测
特异性检测
方便:最小样本处理;快速方案(1-2小时)
性价比高:100次;高通量筛选兼容
经过验证:可用与哺乳动物组织,细胞和体液
靶点 | 货号 | 检测方法 | 检测极限 | 样本类型 | |
代谢物 |
2-Phosphoglycerate | 93- K778 | C/F | 20 pmol | Cells, tissues |
Dihydroxyacetone Phosphate | 93- K673 | F | 50 pmol | Cells, tissues | |
Glucose | 93- K606 | C/F | 100 pmol | Serum, cells, tissues, urine | |
Glucose | 93- K686 | C | 1000 pmol | Serum, cells, tissues, food | |
Glucose | 93- K688 | F | 10 pmol | Serum, cells, tissues, food | |
Glucose-6-phosphate | 93- K657 | C | 1000 pmol | Serum, cells, tissues | |
Glucose-6-phosphate | 93- K687 | F | 5 pmol | Serum, cells, tissues | |
Phosphoenol Pyruvate | 93- K365 | C/F | 100 pmol | Cells, tissues | |
Pyruvate | 93- K609 | C/F | 100 pmol | Cells, tissues | |
酶 |
Aldolase | 93- K665 | C | 100 mU | Cells, tissues |
Enolase | 93- K691 | C/F | 0.04 mU | Cells, tissues | |
GADPH | 93- K680 | C | 10 mU | Cells, tissues | |
Hexokinase | 93- K769 | F | 2 μU | Serum, cells, tissues | |
Hexokinase | 93- K713 | C | --- | Potential Inhibitors | |
Phosphofructokinase | 93- K776 | C | 1 mU | Cells, tissues | |
Phosphoglucose isomerase | 93- K775 | C | 0.1 mU | Serum, cells, tissues | |
Pyruvate kinase | 93- K709 | C/F | 1000 pmol | Cells, tissues | |
Triose Phosphate Isomerase | 93- K670 | C | 4 mU | Cells, tissues | |
辅酶 |
ADP | 93- K356 | C | 1000 pmol | Tissue, cells, PP |
ADP | 93- K355 | C/F | 100 pmol | Tissue, cells, PP | |
ATP | 93- K354 | C/F | 100 pmol | Tissue, cells, PP | |
ADP/ATP Ratio | 93- K255 | L | < 10 pmol | Cells, PP | |
NADH | 93- K338 | F | < 0.5 pmol | Tissue, cells, PP | |
NAD/NADH Ratio | 93- K337 | C | 10 pmol | Tissue, cells, serum, urine |